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Quantitative regional brain water measurement with magnetic resonance imaging in a focal ischemia model
Author(s) -
Lin Weili,
Paczynski Richard P.,
Venkatesan Ramesh,
He Yong Y.,
Powers William J.,
Hsu Chung Y.,
Haacke E. Mark
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380221
Subject(s) - magnetic resonance imaging , nuclear magnetic resonance , water content , ischemia , materials science , spin echo , chemistry , biomedical engineering , analytical chemistry (journal) , medicine , radiology , physics , chromatography , geotechnical engineering , engineering
Therapeutic approaches to cerebral edema require an understanding of both the magnitude and location of changes in brain water content. It is desirable to have a sensitive, accurate means of measuring brain water noninvasively so that effective therapies for cerebral edema in stroke, head trauma, and other conditions can be investigated. In this work, a three‐dimensional magnetic resonance imaging technique that is able to provide both spin density and T 1 simultaneously is described. This method was used to quantitate regional changes in brain water content in a rat model of focal cerebral ischemia. Brain water contents estimated from both relative spin density and relative T 1 measurements made in vivo were compared with ex vivo measurements of relative tissue water content based on the wet‐dry technique. Correlation coefficients of 0.95 and 0.98 were obtained between the wet‐dry measurements and magnetic resonance measurements of T 1 and spin density, respectively. Notably, the slope of the relationship between T 1 and tissue water content changed dramatically after the injection of a paramagnetic contrast agent while precontrast and postcontrast spin density measurements remained essentially invariant. In addition, a plot of absolute spin density (obtained by normalizing spin density from agar gelatin phantoms of different water contents to the spin density of a sample of 100% water) was linearly related to wet‐dry measurements with a slope of 0.99 (R 2 = 0.99).