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Determination of deoxymyoglobin changes during graded myocardial ischemia: An in Vivo 1 H NMR spectroscopy study
Author(s) -
Chen Wei,
Zhang Jianyi,
Eljgelshoven Marcel H. J.,
Zhang Yi,
Zhu XiaoHong,
Wang Chunshen,
Cho Yong,
Merkle Hellmut,
Uĝcurbil Kǎmil
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380206
Subject(s) - in vivo , blood flow , chemistry , nuclear magnetic resonance spectroscopy , ischemia , oxygenation , myocardial ischemia , nuclear magnetic resonance , myoglobin , cardiology , medicine , biochemistry , biology , physics , microbiology and biotechnology , organic chemistry
1 H NMR spectroscopy was used to detect the proximal histidyl N δ , proton signal of deoxymyoglobin from canine hearts in vivo during graded myocardial ischemia. The NMR signal intensity provided an indicator of intracellular oxygenation in myocardium. The relationship between the myocardial blood flow and the deoxymyoglobin concentration was successfully measured during resting, partial, and complete coronary artery occlusion conditions. The results demonstrate the feasibility to detect deoxymyoglobin using a 1 H NMR spectroscopy technique in living hearts for the first time and the possibility to use this technique for investigating myocardial oxidative metabolism nondestructively and repetitively.