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Evidence for 100% 13 C NMR visibility of glucose in human skeletal muscle
Author(s) -
Roussel Ronan,
Carlier Pierre G.,
Wary Claire,
Velho Gilberto,
Bloch Gilles
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910370604
Subject(s) - skeletal muscle , chemistry , plasma glucose , nuclear magnetic resonance spectroscopy , human muscle , carbohydrate metabolism , in vivo , extracellular , nuclear magnetic resonance , medicine , endocrinology , biology , biochemistry , stereochemistry , diabetes mellitus , microbiology and biotechnology , physics
The accuracy of the measurement of total muscle glucose by in vivo 13 C NMR spectroscopy was tested in five normal volunteers during a euglycemic [1‐ 13 C]glucose infusion. The NMR visible concentration calibrated using an external reference was compared with that calculated from plasma glucose concentration, assuming that glucose remained extracellular. The NMR measurement always provided higher values than the calculation from plasma glucose: 0.51 ± 0.035 (mean ± SE) versus 0.38 ± 0.005 mmol/liter of muscle on average. This systematic difference was interpreted as reflecting the presence of muscle glucose‐6‐phospnate, co‐resonating with free glucose. Thus, glucose appeared to be virtually 100% NMR visible in human skeletal muscle.