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High resolution electron paramagnetic resonance imaging of biological samples with a single line paramagnetic label
Author(s) -
Kuppusamy Periannan,
Wang Penghai,
Chzhan Michael,
Zweier Jay L.
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910370402
Subject(s) - electron paramagnetic resonance , hyperfine structure , laser linewidth , resolution (logic) , paramagnetism , nuclear magnetic resonance , line (geometry) , chemistry , spectral line , magnetic resonance imaging , materials science , optics , physics , atomic physics , computer science , condensed matter physics , medicine , laser , geometry , mathematics , astronomy , artificial intelligence , radiology
The application of electron paramagnetic resonance imaging (EPRI) to obtain information from biological samples has been limited by the lack of ideal single line radical labels. The commonly used nitroxides exhibit multiple lines causing either hyperfine‐based limitations in the maximum obtainable image resolution or hyperfine‐based artifacts in the reconstructed image. The use of a novel single‐line triarylmethyl paramagnetic label that enables marked enhancement in image quality and resolution is reported. This label exhibits a single line EPR spectrum that is sharp (linewidth ∼60 mG in the absence of oxygen) and relatively stable in tissues. The potential of this label in enabling high resolution EPR imaging of biological samples was demonstrated in a series of phantoms and isolated biological organs such as the rat kidney. The images demonstrate that resolutions better than 100 μm could be obtained at L‐band on samples of up to 20 mm in size.