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Enhanced resolution of proton NMR spectra of malignant lymph nodes using magic‐angle spinning
Author(s) -
Cheng Leo L.,
Lean Cynthia L.,
Bogdanova Anna,
Wright S. Carter,
Ackerman Jerome L.,
Brady Thomas J.,
Garrido Leoncio
Publication year - 1996
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910360502
Subject(s) - lymph , lymph node , magic angle spinning , nuclear magnetic resonance , chemistry , glutamine , nuclear magnetic resonance spectroscopy , proton , analytical chemistry (journal) , pathology , medicine , amino acid , biochemistry , physics , chromatography , quantum mechanics
Abstract Proton NMR spectroscopy has proven useful in the detection of cancer in lymph node tissue. However, due to the high fat content of this type of tissue, 2D 1 H COSY measurements (requiring acquisition times of 4–5 h or longer) are necessary to obtain the spectral information necessary for diagnosis. T 2 ‐filtered proton magic‐angle spinning (MAS) NMR spectros‐copy provides 1D spectra of lymph nodes in approximately 20 min with sufficient spectral resolution allowing for identification of changes in cellular chemistry due to the presence of malignant cells. MAS data from lymph nodes of five control and six rats with mammary adenocarcinoma (R13762) demonstrated increases in the signal intensity of resonances associated primarily with lactate (s̀ = 4.12 ppm) P < 0.0004, creatines/lysine (s̀ = 3.04 ppm) P < 0.0032, and glutamate/ glutamine (s̀ = 2.36 ppm) P < 0.0002 in metastatic compared with normal lymph nodes. The infiltration of lymph nodes by malignant cells is an important prognostic factor for many cancers. The rapid assessment of node tissue without the introduction of sampling errors (inherent in currently employed histological procedures) would allow postoperative therapy decisions to be made more efficiently.