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Dynamic electron spin resonance (ESR) imaging of the distribution of spin labeled dextran in a mouse
Author(s) -
Kazama Shunsuke,
Takashige Gou,
Yoshioka Hisashi,
Tanizawa Hisayuki,
Ogata Tateaki,
Koscielniak Janusz,
Berliner Lawrence J.
Publication year - 1996
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910360407
Subject(s) - nuclear magnetic resonance , dextran , electron paramagnetic resonance , magnetic resonance imaging , spin (aerodynamics) , site directed spin labeling , distribution (mathematics) , materials science , condensed matter physics , chemistry , physics , medicine , radiology , thermodynamics , biochemistry , mathematical analysis , mathematics
The nitroxide group 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) was covalently bound to a dextran via an N‐acetamido linkage to produce a novel spin probe, TEMPO‐dextran (TEMPO‐DX), which circulates for long time periods in an animal without metabolic degradation. TEMPO‐DX was stable in mice, while small TEMPO analogs quickly disappeared after administration. Since dextran was reduced somewhat in size during synthesis, the resultant spin‐labeled dextran could be excreted through the kidney. A strong L‐band electron spin resonance signal was obtained shortly after Intravenous administration of TEMPO‐DX into the tail vein of a mouse, from which three‐dimensional images of specific organs were calculated. The signal was found to persist for well over 1 h.