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5‐fluorouracil metabolite patterns in viable and necrotic tumor areas of murine colon carcinoma determined by 19 F NMR spectroscopy
Author(s) -
Kamm Yvonne J. L.,
Heerschap Arend,
Rosenbusch Gert,
Rietjens Ivonne M. C. M.,
Vervoort Jacques,
Wagener D. J. Theo
Publication year - 1996
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910360317
Subject(s) - metabolite , nuclear magnetic resonance spectroscopy , chemistry , fluorouracil , metabolism , alanine , perchloric acid , nuclear magnetic resonance , spectroscopy , biochemistry , amino acid , stereochemistry , medicine , chemotherapy , physics , organic chemistry , quantum mechanics
High‐resolution 19 F NMR spectroscopy at 9.4 T was used to study the difference in the metabolite pattern of 5‐fluorouracil (5‐FU) between viable and necrotic tissues of C38 murine colon tumors grown in C57BI/6 mice. Studies were performed on perchloric acid extracts of these tumor fractions after 5‐FU treatment. The 19 F nuclear magnetic resonance spectra exhibited resonances representing 5‐FU, the catabolites α‐fluoro‐β‐ureidopropionic acid and α‐fluoro‐β‐alanine, as well as several fluoronucleotide anabolites. The absolute concentrations of anabolites and catabolites and the anabolite‐to‐catabolite ratio were significantly lower in the necrotic fraction than in the viable tumor fraction 50 min after administration of 5‐FU, whereas the absolute concentration of 5‐FU was the same. Therefore, in 5‐FU metabolism studies with NMR spectroscopy, it is important to consider the necrotic contribution to the tumor volume.