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Localized 1 H NMR spectroscopy of rat spinal cord in Vivo
Author(s) -
Zelaya Fernando O.,
Chalk Jonathan B.,
Mullins Paul,
Brereton Ian M.,
Doddrell David M.
Publication year - 1996
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910350401
Subject(s) - spinal cord , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , spectroscopy , voxel , chemistry , in vivo , phase (matter) , pulse sequence , physics , medicine , biology , neuroscience , microbiology and biotechnology , organic chemistry , radiology , quantum mechanics
A movable, actively decoupled surface coil has been employed to obtain a localized 1 H NMR spectrum from the lumbosacral spinal cord of a live Lewis rat. A volume selective ‘VOSY’ normally spelled out as ‘volume selective spectroscopy’ spectroscopy pulse sequence that incorporates “phase ramped” selective RF pulses, has been used to minimize random phase jitter in the NMR signal as a result of the large frequency shifts required to locate the voxel in the center of the cord while using intense gradient pulses. Spectra from 13‐μl voxels in healthy rats and in rats inoculated with guinea pig spinal cord and complete Freund's adjuvant, resulting in experimental autoimmune encephalomyelitis, are shown.