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Evaluation of intracellular diffusion in normal and globally‐ischemic rat brain via 133 Cs NMR
Author(s) -
Neil Jeffrey J.,
Duong Timothy Q.,
Ackerman Joseph J. H.
Publication year - 1996
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910350310
Subject(s) - intracellular , effective diffusion coefficient , chemistry , diffusion , nuclear magnetic resonance , biophysics , medicine , biochemistry , magnetic resonance imaging , biology , physics , radiology , thermodynamics
The question of whether the apparent diffusion coefficient (ADC) of intracellular water changes after brain injury was addressed by using 133 Cs as an indicator to report on the state of the intracellular environment. Cesium is an NMR‐detect‐able potassium analog that accumulates in the intracellular space and is detectable in rat brain after being added to the animal's diet. The ADC of cesium was measured before and after the death of the rat. The cesium ADC fell from 0.91 ± 0.05 × 10 −3 mm 2 /s (mean ± SEM, n = 5) in the alive rat to 0.71 ± 0.05 × 10 −3 mm 2 /s within 20 min (the best time resolution of the experiment) of the death of the animal and stayed at this value for at least 3 h ( P < 0.001). Assuming that the ADC of cesium reflects motion in the intracellular environment, these results support the idea that there are changes associated with cell injury that would cause a reduction in the ADC of intracellular water. Hence, one factor contributing to the decrease in water ADC after brain injury is a change in the ADC of intracellular water.