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31 P NMR Study of Acute Toxic Effects of Cadmium Chloride on Rat Liver
Author(s) -
Yoshioka Hiroshi,
Itai Yuji,
Mitsumori Fumiyuki
Publication year - 1995
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910330609
Subject(s) - in vivo , chemistry , cadmium , oxidative phosphorylation , cadmium chloride , phosphate , phosphorylation , metabolism , biochemistry , biology , microbiology and biotechnology , organic chemistry
In this study, acute effects of cadmium ions (Cd 2+ ) on energy metabolism in rat livers were analyzed in vivo after intravenous administration using 31 P NMR. Both inorganic phosphate ( P i ) and nucleotide triphosphate (NTP) peaks of in vivo Cdtreated livers gradually decreased over a 6‐h period. In the extract, NTP peaks in Cdtreated livers were lower, as in the in vivo experiments, but the P i peak was significantly higher than the control. The apparent decrease in P i in in vivo liver treated with Cd could be caused by the reduced visibility of P i because of its uptake into mitochondria from cytoplasm, accompanied by the uncoupling of oxidative phosphorylation by Cd 2+ . These results indicated that total P i in the hepatocytes increases after Cd administration. However, only 10% of P i was visible in Cd‐treated livers in vivo , whereas 34% of P i was visible in controls. Significant increases in phosphocholine and glycerophosphocholine were also observed in extracts of Cd‐treated livers.