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Interpretation of 31 P MRS Spectra in Determining Intracellular Free Magnesium and Potassium Ion Concentrations
Author(s) -
Golding Elke M.,
Golding Raymund M.
Publication year - 1995
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910330403
Subject(s) - magnesium , chemistry , equilibrium constant , ionic bonding , spectral line , ion , potassium , resonance (particle physics) , analytical chemistry (journal) , nuclear magnetic resonance , thermodynamics , inorganic chemistry , atomic physics , physics , chromatography , organic chemistry , astronomy
The current paper develops a simple mathematical model for the determination of the concentration of ionic species using the chemical shifts of ATP from phosphorus magnetic resonance spectroscopy ( 31 P MRS) spectra. An iterative method is used to evaluate the equilibrium constants for a range of Mg/ATP equilibria. Initially, an expression for free magnesium concentration is determined by consideration of four equilibria. We then demonstrate how any number of equilibria may be included using this simplified analysis, by incorporation of pseudo equilibrium constants. This approach may be applied to the interpretation of both ex vivo and, more importantly, in vivo MRS spectra. Furthermore, the authors suggest that the commonly used equation for determining free intracellular magnesium concentration as presented by Gupta and colleagues is an oversimplification, particularly under conditions of fluctuating pH.