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Dynamic Monitoring of Total‐Body Absorption by 19 F NMR Spectroscopy: One Hour Ventilation of HFA‐134a in Male and Female Rats
Author(s) -
Finch John R.,
Dadey Eric J.,
Smith Stanford L.,
Harrison Lester I.,
Digenis George A.
Publication year - 1995
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910330314
Subject(s) - ventilation (architecture) , chemistry , in vivo , atmosphere (unit) , plateau (mathematics) , nuclear magnetic resonance , steady state (chemistry) , absorption (acoustics) , analytical chemistry (journal) , materials science , chromatography , biology , mechanical engineering , mathematical analysis , physics , microbiology and biotechnology , mathematics , engineering , composite material , thermodynamics
Six male and six female Sprague‐Dawley rats were ventilated head‐only for 1 h on a 15% atmosphere of 1, 1, 1, 2‐tetrafluoroethane (HFA‐134a) in air in a magnetic resonance imaging spectrometer. Results from these dynamic 19 F NMR studies suggest that a steady‐state in vivo concentration of HFA‐134a was approached at approximately 25 min into the exposure. Quantitative integration analysis using an external standard estimated this plateau to be 58.3 ± 11.9 mg of absorbed HFA‐134a per rat. The HFA‐134a 19 F NMR signal disappeared rapidly following removal of the test atmosphere, with an elimination half‐life of 4.6 ± 0.6 min in the male rats and 4.9 ± 1.5 min in the female rats. The data suggest that there was no statistical difference between the sexes in amount absorbed or in elimination half‐lives.