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Quantitation of lipid in biological tissue by chemical shift magnetic resonance imaging
Author(s) -
Wong WaiFan,
Northrup S. Robert,
Herrick Richard C.,
Glornbicki Alan P.,
Wood R. Patrick,
Morrisett Joel D.
Publication year - 1994
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910320403
Subject(s) - chemistry , lipid metabolism , liver tissue , lipid accumulation , chloroform , in vivo , iodine value , fatty liver , chromatography , biochemistry , biology , pathology , medicine , microbiology and biotechnology , disease , endocrinology
A method combining several previously used approaches is described for the rapid, accurate quantitation of the fat content of biological tissue based on chemical shift images (CSI) corrected for magnetic field inhomogeneity, and compensated for T 1 , and T 2 , effects. The gravimetrically determined lipid content of fatty tissues (pork fat, rabbit and human liver) that had been differentially depleted of lipid by chloroform extraction correlated well (r = 0.99) with the lipid image intensities of the respective tissues. This multi‐point CSI method was used to quantitate lipid in fresh fatty human liver tissue (wet and dry) containing varying amounts of lipid. Plots of integrated lipid intensity versus tissue lipid content gave straight parallel lines for hydrated (r = 0.94) and dehydrated (r = 0.98) tissues, permitting determination of a proportionality constant for measuring absolute amounts of lipid present in a specific biological tissue. These results suggest the feasibility of using the method in vivo for absolute quantitation of lipid in tissues of agricultural (e.g. pork, beef) and medical (e.g. human liver) interest.