In Vivo detection of a boron‐neutron‐capture agent in melanoma by proton observed 1 H ‐ 10 B double resonance

The Cesium salt of BSSB (Cs 4 B 24 H 22 S 2 ), a common boron‐neutron‐capture‐therapy (BNCT) agent, was injected into M2R mouse melanoma xenografts, and detected in vivo by 1 H‐observed, 10 B‐edited NMR spectroscopy. The technique of spin‐echo difference spectroscopy, in which a proton spin‐echo is detected following the alternating presence and absence of a 10 B 180° pulse was used. This method provides much higher sensitivity than direct 10 B NMR detection, and should thus be suitable for in vivo detection in patients about to undergo BNCT treatment, where the infused agents are 95% isotopically enriched in 10 B.