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Studies on bromobenzene‐induced hepatotoxicity using in vivo MR microscopy with surgically implanted RF coils
Author(s) -
Zhou Xiaohong,
Maronpot Robert R.,
Gofer Gary P.,
Hedlund Laurence W.,
Johnson G. Allan
Publication year - 1994
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910310607
Subject(s) - in vivo , bromobenzene , microscopy , magnetic resonance imaging , nuclear magnetic resonance , materials science , biomedical engineering , nuclear medicine , chemistry , pathology , medicine , biology , radiology , physics , biochemistry , microbiology and biotechnology , catalysis
Abstract Using surgically implanted RF coils at 300 MHz, three‐dimensional microscopic MR images of rat liver were obtained in vivo to follow the development of pathology induced by bromobenzene exposure. Formalin fixed specimens of liver from these animals were also imaged using in vitro MR microscopy, followed by conventional optical microscopy. All MR images were acquired using a spin‐warp pulse sequence with TR = 950 ms and TE = 23 ms. The in vivo images were reconstructed as 256 2 × 32 arrays with a voxel size of (50 μm) 2 × 219 pm, while the in vitro images were reconstructed as 256 2 × 128 arrays, giving an isotropic resolution at (39 μm) 2 . Based on results from six animals, we have found in all animals exposed to bromobenzene, image intensity decreased in specific hepatic tissue regions. These regions were well correlated to low signal intensity areas observed in in vitro MR images at higher resolution. Conventional optical microscopy indicated that the low signal intensity regions corresponded to areas of necrosis. The decrease in signal intensity is consistent with increased local diffusion coefficients as a result of necrosis. This study demonstrates that MR microscopy with implanted RF coils can be successfully used to follow tissue pathological changes in living tissues.