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13 C NMR study of the generation of C 2 ‐ and C 3 ,‐deuterated lactic acid by tumoral pancreatic islet cells exposed to D ‐[1‐ 13 C]‐, D ‐[2‐ 13 C]‐ and D ‐[6‐ 13 C]‐Glucose in 2 H 2 O
Author(s) -
Willem Rudolph,
Biesemans Monique,
Kayser François,
Malaise Willy J.
Publication year - 1994
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910310304
Subject(s) - aldolase a , chemistry , glycolysis , triosephosphate isomerase , lactic acid , nuclear magnetic resonance spectroscopy , dihydroxyacetone phosphate , fructose , molecule , deuterium , enzyme , biochemistry , stereochemistry , biology , bacteria , organic chemistry , genetics , physics , quantum mechanics
Tumoral pancreatic islet cells of the RIN5mF line were incubated for 120 min in media prepared in 2 H 2 O and containing D ‐[1‐ 13 C]glucose, and D ‐[2‐ 13 C]glucose, and D ‐[6‐ 13 C]glucose. The generation of C 2 ‐ and C 3 ‐ deuterated lactic acid was assessed by 13 C NMR. The interpretation of experimental results suggests that a) the efficiency of deuteration on the C 1 of D‐fructose 6‐phosphate does not exceed about 47% and 4% in the phosphoglucoisomerase and phosphomannoisomerase reactions, respectively; b) approximately 38% of the molecules of D ‐glyceraldehyde 3‐phosphate generated from D ‐glucose escape deuteration in the sequence of reactions catalyzed by triose phosphate isomerase and aldolase; and c) about 41% of the molecules of pyruvate generated by glycolysis are immediately converted to lactate, the remaining 59% of pyruvate molecules undergoing first a single or double back‐and‐forth interconversion with L ‐alanine. It is proposed that this methodological approach, based on high resolution 13 C NMR spectroscopy, may provide novel information on the regulation of back‐and‐forth interconversion of glycolytic intermediates in intact cells as modulated, for instance, by enzyme‐to‐enzyme tunneling.