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pH control in rat skeletal muscle during exercise, recovery from exercise, and acute respiratory acidosis
Author(s) -
Kemp G. J.,
Thompson C. H.,
Sanderson A. L.,
Radda G. K.
Publication year - 1994
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910310203
Subject(s) - acidosis , respiratory acidosis , skeletal muscle , respiratory system , medicine , cardiology
We used 31 P magnetic resonance spectroscopy to compare the response of rat skeletal muscle to three kinds of proton load. During exercise (tetanic sciatic nerve stimulation), protons from lactic acid were buffered passively and consumed by net hydrolysis of phosphocreatine (PCr). During recovery from exercise , the pH‐dependent efflux of protons produced by PCr resynthesis could be partially inhibited by amiloride or 4,4′‐diisothiocyanostilbene‐2,2′‐disulphonate (DIDS), implicating both sodiudproton and bicarbonatelchloride exchange, but was not inhibited by simultaneous respiratory acidosis. In early recovery, up to 30% of proton efflux was mediated by lactatelproton cotransport. During acute respiratory acidosis at rest, the eventual change in muscle pH was consistent with passive buffering and was unaffected by amiloride or DIDS, implying no significant contribution of proton fluxes.