Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers | Zendy

Ballon D. | Zendy; Mahmood U. | Zendy; Jakubowski A. | Zendy; Koutcher J. A. | Zendy

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Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers

Author(s) -

Ballon D.,

Mahmood U.,

Jakubowski A.,

Koutcher J. A.

Publication year - 1993

Publication title -

magnetic resonance in medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.696

H-Index - 225

eISSN - 1522-2594

pISSN - 0740-3194

DOI - 10.1002/mrm.1910300615

Subject(s) - nuclear magnetic resonance , chemistry , nuclear magnetic resonance spectroscopy , spectral line , proton nmr , spectroscopy , analytical chemistry (journal) , materials science , physics , quantum mechanics , astronomy , chromatography

A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeioblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31 P spectra from an in vivo murine MCa mammary carcinoma yielded a well‐resolved phospharylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31 P spectra from a perfused suspension of RIF‐1 fibrosarcoma cells exhibited a γ‐nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.

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