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Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers
Author(s) -
Ballon D.,
Mahmood U.,
Jakubowski A.,
Koutcher J. A.
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300615
Subject(s) - nuclear magnetic resonance , chemistry , nuclear magnetic resonance spectroscopy , spectral line , proton nmr , spectroscopy , analytical chemistry (journal) , materials science , physics , quantum mechanics , astronomy , chromatography
A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeioblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31 P spectra from an in vivo murine MCa mammary carcinoma yielded a well‐resolved phospharylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31 P spectra from a perfused suspension of RIF‐1 fibrosarcoma cells exhibited a γ‐nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.