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Simultaneous measurement of both lipid and lactate in isolated rat hearts by 1 H NMR spectroscopy
Author(s) -
Stewart Laura C.,
Saunders John K.,
Deslauriers Roxanne,
Bourgeois Dominique,
Nédélec JeanFrançois J.
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300602
Subject(s) - perfusion , chemistry , ischemia , lipid metabolism , medicine , nuclear magnetic resonance spectroscopy , endocrinology , biochemistry , biology , stereochemistry
Myocardial lipid and lactate levels are sensitive indicators of biochemical status: lipid levels have been shown to increase in response to high fat diets, disease or metabolic stress and elevated lactate levels are indicative of reduced oxygen supply. Selective measurement of lactate or lipid levels by 1 H NMR is not straightforward since both the lactate methyl group and lipid methylene groups resonate at 1.3 ppm. We have overcome this difficulty by employing spectral editing techniques to observe both lipid methylene and lactate methyl resonances, and have measured lipid and lactate levels in perfused rat hearts during control perfusion and in response to metabolic stress. Lactate increased during ischemia and decreased during reperfusion, and the ischemia‐induced increase is inhibited by iodoacetate, as expected. In contrast, lipid levels increased during ischemia and remained elevated during reperfusion. Hearts from rats fed high fat diet show elevated lipid levels during control perfusion. Data obtained by 1 H NMR are consistent with biochemical data, validating the technique.