Premium
Comparison of methods for the determination of absolute metabolite concentrations in human muscles by 31 P MRS
Author(s) -
Buchli Reto,
Boesiger Peter
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300505
Subject(s) - calibration , metabolite , homonuclear molecule , imaging phantom , reproducibility , in vivo , chemistry , calibration curve , accuracy and precision , nuclear magnetic resonance , analytical chemistry (journal) , chromatography , mathematics , nuclear medicine , physics , biology , statistics , biochemistry , detection limit , medicine , microbiology and biotechnology , organic chemistry , molecule
In order to determine metabolite concentrations in human skeletal muscles by in vivo 31 P MRS, different quantification methods were analyzed with regard to the accuracy and reproducibility of results and the simplicity of handling. Each quantification method comprised a calibration strategy and a localization technique. Extensive in vivo and in vitro tests showed that homonuclear phantom‐based calibration strategies yielded significantly more accurate (lower systematic errors) and more reproducible (lower statistical errors) concentration estimates than heteronuclear strategies using internal water as a concentration standard. Additionally, the former strategies are easier to handle than the latter. Localization with the volume‐selective sequence lSlS yielded slightly more reproducible results than localization by surface coil. We conclude that phosphorus metabolite concentrations are determined most accurately with phantom‐based calibration strategies in combination with lSlS localization (measurement errors ≈ 5–7%).