Premium
Dynamic 13 C− 1 H nuclear polarization of lipid methylene resonances applied to broadband proton‐decoupled in vivo 13 C MR spectroscopy of human breast and calf tissue
Author(s) -
Ende Gabriele,
Bachert Peter
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300404
Subject(s) - nuclear magnetic resonance , in vivo , proton , nuclear overhauser effect , chemistry , spectral line , methylene , spectroscopy , nuclear magnetic resonance spectroscopy , proton nmr , polarization (electrochemistry) , analytical chemistry (journal) , physics , chromatography , microbiology and biotechnology , organic chemistry , quantum mechanics , astronomy , biology
Dynamic nuclear polarization of the coupled 13 C− l H spin system was studied for optimizing the signal‐to‐noise ratio of in vivo 13 C MR spectra. In particular, the truncated driven and transient nuclear Overhauser effect (NOE) of the proton‐decoupled 13 C resonances from methylene carbons in vegetable oil and in human calf tissue was observed. Maximum in vivo NOE enhancements n = 1.5 and 0.9 were found, respectively. Theoretical fits to the data yield 13 C− 1 cross‐relaxation times in the order of 0.6 s. Significant signal enhancement over the whole in vivo 13 C chemical shift range is obtained with minimum expense utilizing the NOE of the dipolar coupled 13 C− 1 spin system in addition to proton‐decoupling. NOE‐enhanced proton‐decoupled in vivo 13 C MR spectra were acquired within 17 min in volunteer examinations from the human breast and the calf. These spectra show well‐resolved resonances of carbons in lipids and several other cellular compounds.