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Transaminase reaction rates, transport activities and TCA cycle analysis by post‐steady state 13 C NMR
Author(s) -
Robitaille PierreMarie L.,
Rath Dipti P.,
Skinner Thomas E.,
Abduljalil Amir M.,
Hamlin Robert L.
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300218
Subject(s) - citric acid cycle , transaminase , steady state (chemistry) , chemistry , malate dehydrogenase , catalytic cycle , biochemistry , metabolism , enzyme
In this work, we present the post‐steady state analysis of the TCA cycle and a closed form solution to the rate of label washout from the C4 carbon of glutamic acid through the transaminases and the malate‐aspartate shuttle and then through α‐ketoglutarate dehydrogenase. We demonstrate using a model of this problem that the rate of label washout depends not only on the flux through α‐ketoglutarate dehydrogenase, but most importantly on the activity of the malate‐aspartate shuttle as determined by the forward and reverse fluxes through the transaminases and by the rate of transport of glutamate and a‐ketoglutarate across the mitochondrial membrane.