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13 C NMR investigation of synovial fluids
Author(s) -
Albert Klaus,
Michele Sabine,
Günther Ulrich,
Fial Manfred,
Gall Hugo,
Saal Johannes
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910300213
Subject(s) - depolymerization , synovial fluid , hyaluronic acid , degree of polymerization , chemistry , biological fluids , rheumatoid arthritis , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , carbon 13 nmr , nmr spectra database , viscoelasticity , polymerization , spectral line , pathology , materials science , chromatography , medicine , polymer chemistry , polymer , organic chemistry , osteoarthritis , anatomy , physics , alternative medicine , astronomy , composite material
13 C NMR spectra of synovial fluids from 20 patients suffering from rheumatic diseases have been recorded. Structural changes in hyaluronic acid, the main carrier of the viscoelastic properties of synovial fluid, could be observed in the NMR spectra of the native biological fluid. By comparing these spectra with those of purified hyaluronic acid, a rough estimation of the degree of depolymerization of synovial hyaluronlic acid was possible. The patients with rheumatoid arthritis appeared to have a lower degree of polymerization compared to patients with osteoarthrosis. Thus, 13 C NMR spectroscopy prolvides useful information about biophysical properties of sartorial fluid.