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Allergen‐induced histamine secretion associated with lactate production in mast cells detected by 1 H NMR
Author(s) -
Yoshizaki Kazuo,
Arizono Naoki,
Hayano Takashi,
Watari Hiroshi
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910290604
Subject(s) - histamine , chemistry , secretion , melittin , compound 48/80 , allergic response , diamine oxidase , nuclear magnetic resonance spectroscopy , mast cell , biochemistry , immunoglobulin e , microbiology and biotechnology , medicine , degranulation , immunology , endocrinology , biology , stereochemistry , enzyme , antibody , receptor , peptide
Allergen‐induced histamine secretion in mast cells, obtained from rats infected with Nippostrongylus brasiliensis, was detected by a 1 H NMR semi‐selective excitation technique. Lactate production was also detected in association with histamine secretion as observed in mast cells stimulated with other nonimmunological agents, such as compound 48/80, poly‐myxin B, and melittin. The assignment of the lactate resonance was confirmed using lactate oxidase. Thus, 1 H NMR spectroscopy is a useful method to study the activation of mast cells in allergic reaction.