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Imaging oxygen tension in liver and spleen by 19 F NMR
Author(s) -
Holland Scott K.,
Kennan Richard P.,
Schaub Maria M.,
D'Angelo Michael J.,
Gore John C.
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910290405
Subject(s) - oxygen tension , carbogen , chemistry , oxygen , spleen , in vivo , nuclear magnetic resonance , spin–lattice relaxation , mononuclear phagocyte system , nuclear medicine , pathology , medicine , physics , microbiology and biotechnology , organic chemistry , nuclear quadrupole resonance , biology
19 F NMR imaging of the perfluorocarbon emulsion Fluosol TM has been used to study regional variations in oxygen tension in rat liver and spleen. We have used the linear dependence of spin lattice relaxation rate (1/ T 1 ) on the partial oxygen pressure (pO 2 ) of Fluosol TM to determine the oxygen tension in the reticuloendothelial system (RES) of the liver and spleen of male Sprague‐Dawley rats which have serial infusions of Fluosol TM . Oxygen tension maps have been computed from 19 F NMR images using a calibration obtained for Fluosol TM in vitro at 37.5°C. The spatial resolution of the pO 2 maps computed using this technique is 1.2 × 1.2 mm in 3‐mm thick slices. Calculations from in vivo pO 2 maps indicate an average change in the median pO 2 of the RES from 118 to 80 mmHg for ( n = 7) rats breathing 95% O 2 and 5% CO 2 (carbogen) and air, respectively.