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Effect of glucose and confluency on phosphorus metabolites of perfused human prostatic adenocarcinoma cells as determined by 31 P MRS
Author(s) -
Macdonald Jeffrey M.,
Kurhanewicz John,
Dahiya Rajvir,
Espanol Maryceline T.,
Chang LeeHong,
Goldberg Beth,
James Thomas L.,
Narayan Perinchery
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910290213
Subject(s) - cell culture , in vivo , phosphomonoesters , d glucose , chemistry , cell growth , phosphorus , phosphate , cell division , adenocarcinoma , biology , endocrinology , medicine , cell , biochemistry , inorganic phosphate , cancer , genetics , microbiology and biotechnology , organic chemistry
A series of perfused cell 31 P MRS studies were conducted using a well established human prostate adenocarcinoma cell line (DU 145) at different phases of growth, and exposed to varying glucose concentrations during growth. The spectral characteristics of perfused DU 145 cells were compared with the same cells grown in nude mice (xenografts). Perfused DU 145 cells had lower levels of inorganic phosphate and phos‐phocreative relative to in vivo nude mice xenografts. 31 P MR spectra obtained from perfused cells at different phases of growth and exposed to varying glucose concentrations during grown suggest that increases in diphosphodiester levels are associated with high glucose concentrations and confluency. Perfused DU 145 cells grown in 5.5 mm glucose and harvested at log phase of growth best reflected the phosphorus MR spectra of the same cell line grown in nude mice.