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NMR measurement of 39 K detectability and relaxation constants in rat tissue
Author(s) -
Wellard R. Mark,
Shehan B. Philip,
Adam William R.,
Craik David J.
Publication year - 1993
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910290113
Subject(s) - flame photometry , potassium , relaxation (psychology) , chemistry , nuclear magnetic resonance , liver tissue , isotopes of potassium , analytical chemistry (journal) , biology , physics , endocrinology , chromatography , organic chemistry , neuroscience
Differences in the NMR detectability of 39 K in various excised rat tissues (liver, brain, kidney, muscle, and testes) have been observed. The lowest NMR detectability occurs for liver (61 ± 3% of potassium as measured by flame photometry) and highest for erythrocytes (100 ± 7%). These differences in detectability correlate with differences in the measured 39 K NMR relaxation constants in the same tissues. 39 K detectabilities were also found to correlate inversely with the mitochondrial content of the tissues. Mitochondria prepared from liver showed greatly reduced 39 K NMR detectability when compared with the tissue from which it was derived, 31.6 ± 9% of potassium measured by flame photometry compared to 61 ± 3%. The detectability of potassium in mitochondria was too low to enable the measurement of relaxation constants. This study indicates that differences in tissue structure, particularly mitochondrial content are important in determining 39 K detectability and measured relaxation rates.