Premium
Phosphoarginine‐Adenosine Triphosphate Exchange Detected in Vivo in a Microscopic Nematode Parasite by Flow 31 P FT‐NMR Spectroscopy
Author(s) -
Thompson S. N.,
Platzer E. G.,
Lee R.W.K.
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910280213
Subject(s) - nuclear magnetic resonance spectroscopy , adenosine triphosphate , chemistry , in vivo , biophysics , nuclear magnetic resonance , biochemistry , biology , stereochemistry , physics , microbiology and biotechnology
Abstract In vivo flow 31 P NMR spectroscopy of a microscopic nematode, Steinernema carpocapsae , is described. Long‐term viability was maintained during analysis by continuous circulation of an oxygenated suspension of the parasite through an NMR spectrometer. Saturation transfer and inversion recovery were employed under flowing conditions to investigate the kinetics of phosphoarginine ⇌ adenosine triphosphate exchange. The kinetic constants for the forward and reverse reactions were 0.37/s and 1.45/s, respectively. This report is the first to demonstrate a functional phosphagen kinase in the metabolism of a parasitic helminth.© 1992 Academic Press,Inc.