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A noninvasive assessment of myocardial oxygen tension: 19 f nmr spectroscopy of sequestered perfluorocarbon emulsion
Author(s) -
Mason Ralph P.,
Jeffrey F. Mark H.,
Malloy Craig R.,
Babcock Evelyn E.,
Antich Peter P.
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910270210
Subject(s) - emulsion , oxygen tension , oxygen , chemistry , nuclear magnetic resonance spectroscopy , relaxation (psychology) , spectroscopy , nuclear magnetic resonance , medicine , biochemistry , physics , organic chemistry , quantum mechanics
Fluorine NMR spectroscopy of sequestered perfluorocarbon emulsion has been used to measure myocardial oxygen tension. This novel application provides a rapid noninvasive assessment of changes in oxygen tension in response to ischemia and reperfusion. Rats were predosed with Oxypherol‐ET (emulsion of perfluorotributylamine). Following vascular clearance of the emulsion the heart was excised and perfused using the Langendorff retrogradetechnique. 19 F spin‐lattice relaxation time measurements provided an accurate estimate of myocardial p O 2 . Using a two‐point determination with a time resolution of 1 s, the loss of oxygen was found to be complete within 40 s of the onset of global ischemia. The fall in oxygen tension correlated closely with an observed loss of ventricular pressure. Magnetic resonance imaging showed that perfluorocarbon was distributed throughout the heart; thus, this reporter molecule provides a global measurement of oxygen tension.