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Phosphodiesters in saponified extracts of human breast and colon tumors using 31 P magnetic resonance spectroscopy
Author(s) -
Merchant Thomas E.,
Characiejus Dainius,
Kasimos John N.,
Otter Willem Den,
Gierke Louis W.,
Glonek Thomas
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910260114
Subject(s) - saponification , phospholipid , phosphorylcholine , chemistry , phosphatidylethanolamine , phosphodiester bond , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , phosphatidylcholine , pathology , biochemistry , medicine , stereochemistry , membrane , rna , physics , gene
Saponified phospholipid extracts of malignant and normal human breast and colon surgical tissue specimens ( n = 45) generate characteristic phosphodiester profiles using 31 P magnetic resonance (MR) spectroscopy. The resultant 31 P MR spectroscopic profiles of the analyzed tissues are used to differentiate malignant from normal. The appearance of an uncharacterized resonance at 0.29 δ in the malignant tissue spectra (50% of breast and 75% of colon specimens) is the most notable qualitative finding. Quantitatively, malignant colon tissues differ from normal colon tissues with depressed levels of phosphatidylserine and elevated levels of glycerol 3‐phosphorylglycerol and an index measuring the summation of phospholipid polar head group residues with free hydroxyl groups. Malignant breast tissues have significantly elevated levels of glycerol 3‐phosphorylethanolamine and significantly depressed levels of glycerol 3‐phosphorylcholine compared to normal breast tissues, reflecting a perturbation in the balance of lipid residues that are the respective breakdown products of phosphatidylethanolamine and phosphatidylcholine. The concentration of the polar head group residues is compared to 31 P MR spectroscopic profiles of colon and breast tissue phospholipids, in order to demonstrate the quantitative nature of the technique employed.