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The application of 13 C NMR to the characterization of phospholipid metabolism in cells
Author(s) -
Ronen Sabrina M.,
Degani Hadassa
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910250219
Subject(s) - ethanolamine , serine , choline , chemistry , phospholipid , nuclear magnetic resonance spectroscopy , biochemistry , stereochemistry , enzyme , membrane
31 P and 31 C NMR spectroscopy of lipid extracts of T47D human breast cancer spheroids and the use of 13 C‐labeled lipid precursors [3‐ 13 C ] serine, [1,2‐ 13 C] ethanolamine, and [1,2‐ 13 C]choline enabled us to determine the rate of 13 C incorporation into the major phos‐pholipids and to show that the synthesis of phosphatidylethanolarnine in T47D cells is via both the CDP‐ethanolamine pathway and serine decarboxylation, with the extent of each depending on the concentration of ethanolamine in the medium. In the presence of low ethanolamine (3.4 μ M), both pathways contribute in equal proportions, while in the presence of high ethanolamine, the CDP‐ethanolamine pathway predominates. © 1992 Academic Press, Inc.