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1 H NMR study of cortex neurons and cerebellar granule cells on microcarriers and their PCA extracts: Lactate production under hypoxia
Author(s) -
Sonnewald U.,
Petersen S. B.,
Krane J.,
Westergaard N.,
Schousboe A.
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910230117
Subject(s) - granule (geology) , microcarrier , chemistry , cerebellar cortex , cerebral cortex , granule cell , hypoxia (environmental) , cerebellum , cell , biochemistry , neuroscience , biology , dentate gyrus , central nervous system , oxygen , paleontology , organic chemistry
Lactate production of 6‐day‐old cerebral cortex neurons and 7‐day‐old cerebellar granule cells from mouse brain attached to cytodex 3 microcarriers was studied as a function of time, under hypoxic conditions using 1 H NMR. Perchloric acid extracts of both cell types were prepared and 1 H NMR spectra showed compounds characteristic for these neurons. In particular the granule cell extracts showed a large amount of glutamate as expected from biochemical experiments, whereas the cortex neurons showed a large amount of 4‐ aminobutyric acid. © 1992 Academic Press, Inc.