1 H NMR study of cortex neurons and cerebellar granule cells on microcarriers and their PCA extracts: Lactate production under hypoxia | Zendy

Sonnewald U. | Zendy; Petersen S. B. | Zendy; Krane J. | Zendy; Westergaard N. | Zendy; Schousboe A. | Zendy

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1 H NMR study of cortex neurons and cerebellar granule cells on microcarriers and their PCA extracts: Lactate production under hypoxia

Author(s) -

Sonnewald U.,

Petersen S. B.,

Krane J.,

Westergaard N.,

Schousboe A.

Publication year - 1992

Publication title -

magnetic resonance in medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.696

H-Index - 225

eISSN - 1522-2594

pISSN - 0740-3194

DOI - 10.1002/mrm.1910230117

Subject(s) - granule (geology) , microcarrier , chemistry , cerebellar cortex , cerebral cortex , granule cell , hypoxia (environmental) , cerebellum , cell , biochemistry , neuroscience , biology , dentate gyrus , central nervous system , oxygen , paleontology , organic chemistry

Lactate production of 6‐day‐old cerebral cortex neurons and 7‐day‐old cerebellar granule cells from mouse brain attached to cytodex 3 microcarriers was studied as a function of time, under hypoxic conditions using 1 H NMR. Perchloric acid extracts of both cell types were prepared and 1 H NMR spectra showed compounds characteristic for these neurons. In particular the granule cell extracts showed a large amount of glutamate as expected from biochemical experiments, whereas the cortex neurons showed a large amount of 4‐ aminobutyric acid. © 1992 Academic Press, Inc.

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