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In vivo phosphorus NMR spectroscopy of skin using a crossover surface coil
Author(s) -
Chen Yuhchyau,
Richards Todd L.,
Izenberg Seth,
Golden Robert N.,
Williams David L.,
Nelson James A.,
Steen R. Grant
Publication year - 1992
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910230107
Subject(s) - in vivo , phosphocreatine , chemistry , proton nmr , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , phosphate , imaging phantom , nuclear medicine , medicine , biochemistry , biology , stereochemistry , physics , microbiology and biotechnology , energy metabolism
A modified crossover surface coil with minimal B 1 field penetration was used for collection of skin phosphorus NMR spectra. Projection imaging experiments show that the coilsensitive volume is uniform at the phosphorus frequency, but strikingly nonuniform at s the proton frequency. Experiments with an in vitro phosphorus phantom, designed to simulate skin and underlying tissue, demonstrated that 45.1% (±1.2%) of total signal was derived from Sprague‐Dawley rat skin and 19.3% (±1.4%) of total signal was derived from Fischer‐344 rat skin. 31 P MR spectra of rat skin in vivo permitted resolution of four phosphorus compounds: nucleoside triphosphates, phosphocreatine (PCr), inorganic phosphate (P i ), and phosphomonoester. Spectra collected after skin flap surgery in Fischer‐344 rats showed a 50.1% (±7.6%) reduction in the ratio of PCr/P i within 30 min of surgery, compared to presurgical PCr/P i levels (P < 0.01). Skin phosphorus spectra are potentially useful for assessment of skin flap and skin graft viability. © 1992 Academic Press, Inc.