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Determination of cytosolic ADP and AMP concentrations and the free energy of ATP hydrolysis in human muscle and brain tissues with 31 P NMR spectroscopy
Author(s) -
Roth K.,
Weiner M. W.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910220258
Subject(s) - adenylate kinase , atp hydrolysis , creatine kinase , chemistry , creatine , phosphocreatine , pi , energy charge , hydrolysis , cytosol , adenosine triphosphate , nuclear magnetic resonance spectroscopy , human brain , human muscle , adenine nucleotide , biochemistry , enzyme , nucleotide , energy metabolism , biology , skeletal muscle , stereochemistry , endocrinology , atpase , neuroscience , gene
Based on experimentally determined concentrations of PCr, P i , ATP, and H + , the ADP and AMP concentrations and the free energy for the ATP hydrolysis in normal human brain and muscle were calculated assuming that the creatine kinase‐ and the myokinase‐catalyzed reactions were in equilibrium, accounting for changes in pH and free Mg 2+ ion concentration. © 1991 Academic Press, Inc.