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A lineshape fitting model for 1 H NMR spectra of human blood plasma
Author(s) -
Hiltunen Y.,
AlaKorpela M.,
Jokisaari J.,
Eskelinen S.,
Kiviniitty K.,
Savolainen M.,
Kesäniemi Y. A.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910210207
Subject(s) - nuclear magnetic resonance , spectral line , nmr spectra database , chemistry , physics , astronomy
A lineshape fitting model was constructed for classifying the overlapping information in the 1 H NMR spectrum of human blood plasma. A reliable assignment of the overlapping fatty acid (‐CH 2 ‐) n and ‐CH 3 resonances of the various lipoproteins (VLDL, very low density lipoprotein; LDL, low density lipoprotein; HDL high density lipoprotein) is introduced, and for the first time detailed characteristics (chemical shifts, half linewidths, and relative intensities) of the individual lipoprotein components were obtained directly from the whole plasma spectrum. This was achieved by combining the constructed lineshape fitting model and the proper 400 MHz proton NMR measurements from blood plasma of a healthy donor, from fractions of the different lipoproteins, and from plasma samples in which the lipoprotein fractions were separately added. The results suggest fair promise of future applications of the rapid and easy NMR analysis of lipoprotein distribution in various research and clinical situations. © 1991 Academic Press, Inc.