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Evaluation of energy metabolism in brain using epimerization of 2‐deoxy‐2‐fluoro‐d‐glucose by 19 F NMR: The effect of anesthesia
Author(s) -
Shinohara Shunji,
Kanazawa Yoko,
Kojima Masaharu
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910210204
Subject(s) - epimer , metabolism , chemistry , phosphate , nuclear magnetic resonance spectroscopy , energy metabolism , carbohydrate metabolism , anesthetic , nuclear magnetic resonance , biochemistry , endocrinology , medicine , stereochemistry , anesthesia , physics
The effect of anesthesia on the epimerization of 2‐deoxy‐2‐fluoro‐D‐glucose(‐6‐phosphate) to 2‐deoxy‐2‐fluoro‐D‐mannose(6‐phosphate) in mice was demonstrated with 19 F NMR spectroscopy of excised tissue specimens from heart, muscle, and brain. Spectra from each of six excised organs confirmed that pretreatment with an anesthetic suppressed the conversion ratio, FDM/(FDG + FDM), in brain at 3 h from 52 ± 5 to 30 ± 6% under the dose of 100 mg/kg. We propose potential applications for NMR monitoring of FDG‐FDM conversion, including the assessment of regional brain metabolism and the evaluation of Various pharmacologic agents. © 1991 Academic Press, Inc.