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Measurements of pertinent concentrations of oxygen in vivo
Author(s) -
Swartz H. M.,
Boyer S.,
Gast P.,
Glockner J. F.,
Hu H.,
Liu K. J.,
Moussavi M.,
Norby S. W.,
Vahidi N.,
Walczak T.,
Wu M.,
Clarkson R. B.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910200217
Subject(s) - oxygen , in vivo , radical , chemistry , limiting oxygen concentration , biochemistry , biology , organic chemistry , microbiology and biotechnology
Abstract A new method able to measure the concentration of oxygen in complex biological systems, including in vivo , has been developed using low‐frequency EPR and newly characterized free radicals that are very sensitive to the concentration of oxygen. The free radicals (fusinite and lithium phthalocyanine) are very stable in tissues (for at least 150 days), apparently nontoxic, and can reflect oxygen concentrations that are less than the K m of cytochrome oxidase (0.1 μ M or lower). Their biological stability is indicated by the fact that repeated measurements with fusinite of the concentration of oxygen in skeletal muscle have been made in the same animal for more than 150 days without any change in sensitivity or signs of toxicity. © 1991 Academic Press, Inc.