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An NMR probe to study function and metabolism simultaneously in isolated human cardiac tissue
Author(s) -
Lareau Sylvain,
Moore Rayad,
Mainwood Graham W.,
Labow Rosalind S.,
Keon Wilbert J.,
Deslauriers Roxanne
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910200214
Subject(s) - nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , chemistry , nmr spectra database , biophysics , anatomy , spectral line , medicine , biology , physics , astronomy
Abstract Trabeculae isolated from human atrial appendages have been used to study preservation of donor hearts for cardiac transplantation. We have developed a perifusion system equipped with a fiber optic strain gauge to study mechanical performance of human atrial trabeculae (10‐20 mg) while simultaneously observing the energetic compounds by 31 P NMR spectroscopy. The NMR probe consists of an eight‐turn solenoid coil (2.3 mm i. d. × 5 mm length, 24‐gauge wire) double tuned to allow observation of 1 H and 31 P nuclei. The probe and the perifusion system are temperature regulated and permit preservation studies using a variety of small muscles at low temperatures (down to 4°C) as well as at physiological temperature (37°C). 31 P NMR spectra suitable for quantification can be obtained from ≈ 10 mg of human atrial trabeculae in 15 min. Spectra of 8‐to 12‐mg mouse extensor digitalis longus or soleus muscle can be obtained in less than 10 min. © 1991 Academic Press, Inc.