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Fucose in 1 H COSY spectra of plasma membrane fragments shed from human malignant colorectal cells
Author(s) -
Lean Cynthia L.,
Mackin Wanda B.,
Mountford Carolyn E.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910200213
Subject(s) - fucose , hydrolysis , chemistry , acid hydrolysis , threonine , membrane , biochemistry , enzyme , galactose , serine
The methyl‐methine cross peak of bound fucose has been assigned in the COSY spectrum of plasma membrane shed from human malignant colorectal cells. This cross peak (1.33‐4.27 ppm), which is superimposed on the methyl‐methine cross peak of threonine, was assigned following hydrolysis of the sample. Acid hydrolysis led to a 28 ± 5% reduction in the intensity of the cross peak and the apperance of the α and ß forms of fucose. Chemical analysis confirmed the release of free fucose. Lactate anion. which was not perturbed by the hydrolysis, was used as an internal standard. © 1991 Academic Press, Inc.