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Evaluation of ba1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy
Author(s) -
Sostman H. Dirk,
Rockwell Sara,
Sylvia Avis L.,
Madwed David,
Cofer Gary,
Charles H. Cecil,
NegroVilar Rosa,
Moore Deborah
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910200208
Subject(s) - oxygenation , radiosensitivity , chemistry , rhabdomyosarcoma , hemoglobin , deoxygenated hemoglobin , oxygen , microelectrode , methemoglobin , irradiation , carbogen , spectroscopy , nuclear magnetic resonance , analytical chemistry (journal) , pathology , chromatography , electrode , medicine , biochemistry , physics , sarcoma , organic chemistry , nuclear physics , quantum mechanics
Abstract We studied tumor tissue oxygenation in the BA1112 rhabdomyosarcoma using microelectrode pO 2 measurements, optical spectrophotometry, analyses of cell survival after irradiation, and 31 P magnetic resonance spectroscopy (MRS). Studies were carried out in WAG/Rij/Y rats breathing normoxic, hypoxic, and hyperoxic gas mixtures with and without iv administration of perfluorochemical. Significant changes in tissue oxygenation and metabolic status were found when pO 2 values, optical measurements of hemoglobin saturation and cytochrome a, a 3 reduction‐oxidation, radiation cell survival determinations, and MRS measurements of phosphometabolite ratios were obtained in rats breathing different gas mixtures. Inhalation of 100% O 2 caused increases in tumor pO 2 , hemoglobin saturation, cytochrome a, a 3 oxidation, tumor radiosensitivity, and PCr/ P i, NTP/ P i , and PDE/ P i ratios. Such changes were augmented by pretreatment with iv perfluorochemicals. Inhalation of hypoxic gas mixtures resulted in reductions in the above paramenters. These results indicate that tissue oxygenation can be manipulated reproducibly in the BA1112 rhabdomyosarcoma and suggest that 31 P MRS can be used to monitor changes in tumor oxygenation in this model system.