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Dynamic changes in 13 C NMR spectra of intact hearts under conditions of varied metabolite enrichment
Author(s) -
Lewandowski E. Douglas,
Hulbert Chrys
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910190118
Subject(s) - metabolite , chemistry , nuclear magnetic resonance spectroscopy , nmr spectra database , isotope , nuclear magnetic resonance , spectral line , stereochemistry , biochemistry , physics , quantum mechanics , astronomy
Dynamic changes in 13 C NMR signal from enriched glutamate pools within hearts have been examined under varied conditions of metabolite pool size and fractional enrichment. Relative signal intensities of 13 C‐enriched glutamate isotope isomers were similar within spectra from both intact hearts and corresponding in vitro samples. The parameters used to assess metabolic activity with 13 C NMR proved independent of fractional enrichment and pool size. The data show the importance of acknowledging unlabeled, 13 C NMR invisible metabolites. © 1991 Academic Press, Inc.