Premium
In vivo NMR, biochemical, and histologic evaluation of alcohol‐induced fatty liver in rat and a comparison with CCl 4 hepatotoxicity
Author(s) -
Hazle John D.,
Narayana Ponnada A.,
Dunsford Harold A.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910190112
Subject(s) - fatty liver , in vivo , chemistry , ethanol , ex vivo , lipid metabolism , medicine , endocrinology , in vitro , biochemistry , biology , microbiology and biotechnology , disease
Magnetic resonance imaging (MRI) and spectroscopy (MRS) were used to follow the time course of ethanol‐induced fatty liver in a group of 10 rats fed a diet containing 12% alcohol (ethanol) over a 5‐week period. The MR data consisted of T 1 ‐weighted images, in vivo 1 H spectra, and in vivo T 1 , relaxation measurements. Changes in short TR images as a result of fatty accumulation were noted only as a slight increase in liver intensity relative to surrounding muscle. A poorly correlated (r = 0.54) increase in water T 1 with time was observed. No statistically significant changes in lipid T 1 were found. MRS derived lipid content was compared with biochemically derived total lipids and histology. MRS determined liver lipids were found to increase linearly with time ( r = 0.91). Biochemically derived lipid content also increased with prolonged exposure to ethanol ( r = 0.96). The averages of MRS derived lipid content agreed well with the average changes in biochemically determined total lipid concentration. Histologic examination revealed slight to moderate changes in fatty accumulation with significant variation in the group at the end of the study. On an individual basis the MRS and histologic evaluation were highly correlated ( r = 0.94). © 1991 Academic Press, Inc.