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Relative volume‐average murine tumor blood flow measurement via deuterium nuclear magnetic resonance spectroscopy
Author(s) -
Mattiello James,
Evelhoch Jeffrey L.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910180207
Subject(s) - nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , volume (thermodynamics) , deuterium , spectroscopy , blood flow , magnetic resonance imaging , chemistry , nuclear physics , physics , medicine , radiology , quantum mechanics
A deuterium NMR spectroscopic method to determine relative tumor blood flow (TBF) by measuring the increase in tumor HOD concentration after intravenous injection of 100 μl D 2 O (0.9% NaCl) is presented. An integration approach analogous to that validated for positron emission tomographic measurement of cerebral blood flow was implemented. Computer simulations indicated that integration from 30 to 120 s minimizes the sensitivity of the uptake integral to the shape of the arterial input function, which cannot be assessed in each mouse, while maintaining both a nearly linear relationship between TBF and the integral and high NMR signal‐to‐noise. A strong positive linear correlation was observed between the uptake integral and TBF measured by D 2 O clearance in both untreated tumors ( n = 19; P < 0.001) and tumors after hyperthermia ( n = 16; P < 0.001). This method can measure relative TBF in tumors with heterogeneous blood flow and is ideally suited to concurrent or interleaved measurement of TBF and metabolism via multinuclear NMR spectroscopy. © 1991 Academic Press. Inc.