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Analysis of 23 Na NMR spectra from isolated perfused hearts
Author(s) -
Kohler Susan J.,
Perry Stanton B.,
Stewart Laura C.,
Atkinson David E.,
Clarke Kieran,
Ingwall Joanne S.
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910180104
Subject(s) - spectral line , nmr spectra database , perfusion , chemistry , nuclear magnetic resonance , ischemia , intracellular , guinea pig , medicine , biochemistry , physics , astronomy
Abstract The 23 Na NMR spectra obtained from isolated hearts perfused with buffer containing the paramagnetic shift reagent dysprosium triethylenetetraminehexaacetic acid, Dy(TTHA) 3− , are complex and contain a number of overlapping peaks of different intensities. Spectra from rat, rabbit, guinea pig, and ferret hearts obtained during periods of control perfusion are similar and undergo similar changes when the hearts are subjected to periods of ischemia and reflow. The contributions from the intracellular, interstitial, vascular, and bath compartments to the multiple peaks in the spectra of rat hearts have been assigned. The significant contributions to these spectra of bulk magnetic susceptibility effects and incomplete mixing have been demonstrated through a series of modeling experiments. Since the spectra from hearts of different species are so similar, the peak assignments made for the rat are applicable to spectra from rabbit, guinea pig, and ferret hearts as well. This work provides a framework for quantitative analysis of the spectral changes which occur during conditions such as ischemia and reflow. © 1991 Academic Press. Inc.