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Analysis of diabetic cataractogenesis using chemical‐shift nuclear magnetic resonance microscopy
Author(s) -
Cheng HongMing,
Aguayo James B.,
Moore Gregory J.,
Mattingly Mark
Publication year - 1991
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910170111
Subject(s) - nuclear magnetic resonance , magnetic resonance imaging , microscopy , magnetic resonance force microscopy , chemistry , materials science , medicine , physics , pathology , radiology , ferromagnetic resonance , magnetization , magnetic field , quantum mechanics
We here report the analysis of single rabbit lenses under high‐glucose stress using four‐dimensional 13 C nuclear magnetic resonance (NMR) spectroscopy (three spatial and one chemical‐shift). We have produced spatial maps of lenticular metabolites (glucose, sorbitol, and lactate) with submillimeter in‐plane resolution. The production of sorbitol and its inhibition are also presented. This is the first study to report regional tissue metabolism. We expect further improvements in spatial resolution and acquisition times that will enable localized kinetic studies in intact tissues. © 1991 Academic Press, Inc.