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NMR visibility of 39 K and 35 Cl in erythrocytes and kidney tubules
Author(s) -
Boulanger Y.,
Vinay P.
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910160206
Subject(s) - chemistry , intracellular , nuclear magnetic resonance , visibility , chloride , hematocrit , kidney , analytical chemistry (journal) , chromatography , medicine , physics , biochemistry , optics , organic chemistry
The NMR visibility of 39 K and 35 Cl has been investigated in erythrocytes and in dog renal tubules. In erythrocytes, the 39 K NMR visibility was determined by comparing the signal intensities before and after hemolysis with water and by comparing the NMR and flame photometry results. Both procedures showed a NMR visibility of 100% for intracellular potassium. The visibility of intracellular chloride in erythrocytes was estimated at 40% by monitoring the intensity of the 35 Cl signal as a function of the hematocrit value. In the case of kidney proximal tubules, the 39 K visibility appeared to be very low but could not he accurately determined due to the low sensitivity of the nucleus. The 35 Cl signals for intracellular chloride in renal tubules were too broad to be detected. © 1990 Academic Press, Inc.