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Application of reverse‐DEPT polarization transfer pulse sequence to study the metabolism of carbon‐13‐labeled substrates in perfused organs by 1 H NMR Spectroscopy
Author(s) -
Desmoulin F.,
ConfortGouny S.,
Masson S.,
Bernard M.,
Doddrell D. M.,
Cozzone P. J.
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910150311
Subject(s) - dept , metabolism , pulse sequence , nuclear magnetic resonance spectroscopy , chemistry , metabolic pathway , nuclear magnetic resonance , biochemistry , stereochemistry , physics
Metabolism of 13 C‐enriched metabolites can be advantageously studied by reverse‐polarization transfer methods. In this work an improved reverse‐DEPT sequence has been applied for the first time on perfused organs in a 20‐mm probe. The metabolic fate of 99% enriched [ 2‐ 13 C] acetate perfused in excised rat liver and heart has been documented. © 1990 Academic Press, Inc.