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The effect of cardiopulmonary bypass on brain and heart metabolism: A 31 P NMR study
Author(s) -
Swain Julie A.,
Robbins Robert C.,
Balaban Robert S.,
McDonald Thomas J.,
Schneider Benjamin,
Groom Robert C.
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910150310
Subject(s) - phosphocreatine , cardiopulmonary bypass , intracellular ph , energy metabolism , nuclear magnetic resonance spectroscopy , adenosine triphosphate , metabolism , chemistry , inorganic phosphate , intracellular , medicine , cardiology , phosphate , biochemistry , organic chemistry
The development of a large animal preparation using 31 P nuclear magnetic resonance (NMR) spectroscopy for the study of cerebral and myocardial metabolism during cardiopulmonary bypass (CPB) is reported. The effect of normothermic CPB on myocardial and cerebral metabolism was evaluated. Adolescent sheep were used which have low levels of 2,3 ‐diphosphoglycerate, a compound which can interfere with the calculation ofintra‐cellular pH and inorganic phosphate content. CPB was performed using standard procedures modified for the presence of a high magnetic field and limited access to the animal. High quality 31 P NMR data were obtained from the brains and hearts of these animals before and during normothermic CPB. These results demonstrate that the initiation of normothermic CPB does not change high energy phosphate levels or intracellular pH. In particular, the decreased myocardial oxygen demand associated with CPB is not associated with improvement in the levels of adenosine triphosphate or phosphocreatine. The measurements of energy metabolism and intracellular pH of the brain and heart during CPB were possible within the constraints of the NMR experiment without compromising the CPB procedure. Combining NMR and CPB techniques permits future studies of cerebral and myocardial metabolism, especially those relating to ischemia. © 1990 Academic Press, Inc.