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High‐resolution NMR spectroscopy of cerebral white matter in multiple sclerosis
Author(s) -
SappeyMarinier Dominique
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910150206
Subject(s) - white matter , multiple sclerosis , chemistry , nuclear magnetic resonance spectroscopy , myelin , spectroscopy , nuclear magnetic resonance , t2 relaxation , high resolution , relaxation (psychology) , analytical chemistry (journal) , chromatography , magnetic resonance imaging , central nervous system , medicine , radiology , physics , remote sensing , organic chemistry , quantum mechanics , psychiatry , geology
Multiple sclerosis (MS) lesions have been shown by conventional methods to have major alterations in water and myelin lipid contents. To characterize these abnormalities more efficiently, NMR spectroscopy was used to evaluate water content by measuring relaxation times at 0.5 and 2.0 T. Subsequently, cholesterol content was obtained by using 1 H NMR spectroscopy, while 31 P NMR spectroscopy allowed measurement of the four main phospholipids and the inorganic phosphate concentrations in normal and pathological cerebral white matter samples. The relaxation times were significantly prolonged in MS lesions relative to normal white matter. Measured in different sample types, T 1 and T 2 times increased with water content. Moreover, the T 2 of normal‐appearing white matter was considerably lengthened. Analysis of white matter lipid composition using this method gave accurate values, which showed a significant decrease in phospholipids and cholesterol content in MS samples. © 1990 Academic Press, Inc.