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In vivo MRS measurement of deoxymyoglobin in human forearms
Author(s) -
Wang Zhiyue,
Noyszewski Elizabeth A.,
Leigh John S.
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910140314
Subject(s) - in vivo , chemistry , myoglobin , ischemia , human muscle , nuclear magnetic resonance , anatomy , nuclear medicine , skeletal muscle , medicine , biochemistry , biology , physics , microbiology and biotechnology
In vivo MRS measurement of deoxymyoglobin (deoxy‐Mb) in human forearms was performed by observing the N‐δ proton of F8 proximal histidine. The concentration of deoxy‐Mb reflects the oxygen level in the muscle. In resting muscles, the deoxy‐Mb level was below the detection sensitivity. When ischemia was introduced by arterial occlusion, the time‐resolved NMR spectra (with 1 min resolution) was recorded to follow the change in the deoxy‐Mb signal. The result shows that deoxy‐Mb signal builds up and levels off within 6 min. This technique may be used to study bioenergetics of muscle during exercise and under pathological conditions. © 1990 Academic Press, Inc.